Investigators often work with complex nucleic acid samples in procedures such as amplification, renaturation, cDNA synthesis and normalization, sonication, digestion, nicking, labeling and ...
Successfully mastering agarose gel electrophoresis has become an essential skill for most molecular biologists. Done well, the technique can help us diagnose disease, separate desirable from ...
Figure 1: Titration of a 214-bp lac promoter DNA fragment with Escherichia coli CAP protein. Footprinting assays require simultaneous optimization of binding by the protein(s) of interest and the ...
Pulsed-Field Gel Electrophoresis (PFGE) PFGE was developed in 1984 and has since become the gold standard for bacterial subtyping. PFGE is the primary subtyping method used by PulseNet, a network of ...
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